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antibodies against fluorescein isothiocyanate fitc conjugated cd44  (Miltenyi Biotec)


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    Miltenyi Biotec antibodies against fluorescein isothiocyanate fitc conjugated cd44
    Antibodies Against Fluorescein Isothiocyanate Fitc Conjugated Cd44, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+fitc+antibodies/CD44+Antibody%2C+anti-human/pm42142698-116-47-56
    Average 95 stars, based on 148 article reviews
    antibodies against fluorescein isothiocyanate fitc conjugated cd44 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Erratum: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. Instruments and reagents The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    CCK-8 Assay:

    Article Title: Erratum: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. Instruments and reagents The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    TUNEL Assay:

    Article Title: Erratum: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. Instruments and reagents The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Bioprocessing:

    Article Title: Erratum: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. Instruments and reagents The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Labeling:

    Article Title: Erratum: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. Instruments and reagents The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..

    Article Title: Asiaticoside inhibits epithelial-mesenchymal transition and stem cell-like properties of pancreatic cancer PANC-1 cells by blocking the activation of p65 and p38MAPK
    Article Snippet: .. The CO 2 incubator and automatic microplate reader were purchased from Thermo Scientific; flow cytometer was purchased from FASCAN Becton Dickison, USA; vernier calipers (precision 0.1 mm, EC-200) were purchased from Chengdu Measuring Tool & Cutting Tool Co., Ltd.; pancreatic cancer PANC-1 cells were purchased from the cell bank of the Shanghai Chinese Academy of Sciences; ATS was purchased from Guangxi Changzhou Natural Products Development Co., Ltd., with a purity of >95%; RPMI 1640 medium and mycoplasma-free fetal bovine serum were purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; CCK-8 detection kit and TUNEL tissue apoptosis detection kit were purchased from Shenyang Wanlei Biotechnology Co., Ltd.; β-actin monoclonal antibody was purchased from Beijing Soleibao Technology Co., Ltd.; CD133-PE and CD44-FITC antibodies were purchased from Miltenyi Biotec, Germany; rabbit anti-human E-cadherin, N-cadherin, vimentin, and p65, p-p65, p38 mitogen-activated protein kinase (p38MAPK), p-p38MAPK monoclonal antibodies, were purchased from Santa Cruz, USA; horseradish peroxidase (HRP) labeled goat anti-rabbit IgG was purchased from DAKO, Denmark. ..



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    a Flow cytometric analysis of anti-PD-L1-stained cells in tumor tissues after different treatments ( n = 5 independent mice). b Quantification of the percentage of PD-L1 positive cells in ( a ) ( n = 5 independent mice). c Schematic diagram of the mechanism of Sv@PM-M2p-mediated upregulation of PD-L1 within HCC TME. d Schematic illustration of the research procedure in bilateral subcutaneous HCC mouse model. e Representative bioluminescence images of bilateral subcutaneous HCC mice at specific time points with different treatments ( n = 5 independent mice). f Relative bioluminescence intensity curves for the various treatment groups ( n = 5 independent mice). g Flow cytometric analysis of anti-F4/80/CD206-stained macrophages and anti-F4/80/CD86-stained macrophages in tumor tissues after different treatments ( n = 5 independent mice). h Quantification of the percentage of F4/80 + CD206 + M2 TAMs and F4/80 + CD86 + M1 TAMs in ( g ) ( n = 5 independent mice). i Flow cytometric analysis of <t>anti-CD44/CD62L-stained</t> T cells (gated on CD3 + CD8 + T cells) in the spleens after different treatments ( n = 5 independent mice). j Quantification of the percentage of <t>CD44</t> + CD62L - memory T cells in ( i ) ( n = 5 independent mice). k The assay of IFN-γ, TNF, IL-6, and IL-12 in serum after different treatments ( n = 5 independent mice). Unless specified otherwise, error bars represent the mean ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s test ( b , f , h , j , k ) and P -values were indicated. Source data are provided as a Source Data file. The elements in Fig. 8c, d were created by Adobe Illustrator.
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    Image Search Results


    a Flow cytometric analysis of anti-PD-L1-stained cells in tumor tissues after different treatments ( n = 5 independent mice). b Quantification of the percentage of PD-L1 positive cells in ( a ) ( n = 5 independent mice). c Schematic diagram of the mechanism of Sv@PM-M2p-mediated upregulation of PD-L1 within HCC TME. d Schematic illustration of the research procedure in bilateral subcutaneous HCC mouse model. e Representative bioluminescence images of bilateral subcutaneous HCC mice at specific time points with different treatments ( n = 5 independent mice). f Relative bioluminescence intensity curves for the various treatment groups ( n = 5 independent mice). g Flow cytometric analysis of anti-F4/80/CD206-stained macrophages and anti-F4/80/CD86-stained macrophages in tumor tissues after different treatments ( n = 5 independent mice). h Quantification of the percentage of F4/80 + CD206 + M2 TAMs and F4/80 + CD86 + M1 TAMs in ( g ) ( n = 5 independent mice). i Flow cytometric analysis of anti-CD44/CD62L-stained T cells (gated on CD3 + CD8 + T cells) in the spleens after different treatments ( n = 5 independent mice). j Quantification of the percentage of CD44 + CD62L - memory T cells in ( i ) ( n = 5 independent mice). k The assay of IFN-γ, TNF, IL-6, and IL-12 in serum after different treatments ( n = 5 independent mice). Unless specified otherwise, error bars represent the mean ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s test ( b , f , h , j , k ) and P -values were indicated. Source data are provided as a Source Data file. The elements in Fig. 8c, d were created by Adobe Illustrator.

    Journal: Nature Communications

    Article Title: Co-delivery of sorafenib and an FSP1 inhibitor triggers dual ferroptosis in tumor cells and immunosuppressive macrophages for enhanced immunotherapy in mouse models of hepatocellular carcinoma

    doi: 10.1038/s41467-025-65056-9

    Figure Lengend Snippet: a Flow cytometric analysis of anti-PD-L1-stained cells in tumor tissues after different treatments ( n = 5 independent mice). b Quantification of the percentage of PD-L1 positive cells in ( a ) ( n = 5 independent mice). c Schematic diagram of the mechanism of Sv@PM-M2p-mediated upregulation of PD-L1 within HCC TME. d Schematic illustration of the research procedure in bilateral subcutaneous HCC mouse model. e Representative bioluminescence images of bilateral subcutaneous HCC mice at specific time points with different treatments ( n = 5 independent mice). f Relative bioluminescence intensity curves for the various treatment groups ( n = 5 independent mice). g Flow cytometric analysis of anti-F4/80/CD206-stained macrophages and anti-F4/80/CD86-stained macrophages in tumor tissues after different treatments ( n = 5 independent mice). h Quantification of the percentage of F4/80 + CD206 + M2 TAMs and F4/80 + CD86 + M1 TAMs in ( g ) ( n = 5 independent mice). i Flow cytometric analysis of anti-CD44/CD62L-stained T cells (gated on CD3 + CD8 + T cells) in the spleens after different treatments ( n = 5 independent mice). j Quantification of the percentage of CD44 + CD62L - memory T cells in ( i ) ( n = 5 independent mice). k The assay of IFN-γ, TNF, IL-6, and IL-12 in serum after different treatments ( n = 5 independent mice). Unless specified otherwise, error bars represent the mean ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s test ( b , f , h , j , k ) and P -values were indicated. Source data are provided as a Source Data file. The elements in Fig. 8c, d were created by Adobe Illustrator.

    Article Snippet: PerCP/Cyanine5.5 anti-mouse F4/80 (1:200, #E-AB-F0995J), APC anti-mouse CD206 (1:200, #E-AB-F1135E), PE/Cyanine7 anti-mouse CD86 (1:200, #E-AB-F0994H), Fluor Red 780 anti-mouse CD80 (1:200, #E-AB-F0992S), APC anti-mouse CD11c (1:200, #E-AB-F0991E), Fluor Violet 450 anti-mouse CD3 (1:200, #E-AB-F1013Q), Fluor Red 780 anti-mouse CD4 (1:200, #E-AB-F1097S), PerCP/Cyanine5.5 anti-mouse CD8 (1:200, #E-AB-F1104J), FITC anti-human/mouse CD44 (1:200, #E-AB-F1100C), PE anti-mouse Foxp3 (1:200, #E-AB-F1238D), FITC anti-mouse MHC II (1:200, #E-AB-F0990C), APC anti-mouse CD62L (1:200, #E-AB-F1011E), APC anti-mouse PD-L1 (1:200, #E-AB-F1132E), PerCP anti-human CD45 (1:200, #E-AB-F1137F), Fluor647 anti-human CD68 (1:200, #E-AB-F1299M), FITC anti-human CD206 (1:200, #E-AB-F1161C), PE anti-human CD80 (1:200, #E-AB-F1232D), APC anti-human HLA-DR (1:200, #E-AB-F1111E), PE anti-human CD11c (1:200, #E-AB-F1118D), APC anti-human CD3 (1:200, #E-AB-F1001E), and FITC anti-human CD8 (1:200, #E-AB-F1110C) were purchased from Elabscience (Wuhan, China).

    Techniques: Staining